Volume 7 Number 12 (Dec. 2012)
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JCP 2012 Vol.7(12): 2868-2875 ISSN: 1796-203X
doi: 10.4304/jcp.7.12.2868-2875

A Rapid Quantitative Determination Method of AFP Concentration with Gold Immunochromatographic Strip

Haiyan Jiang1, Min Du2, Dongzhong Ke2
1College of Electrical Engineering and Automation, Fuzhou University, Fuzhou, China
2College of Physics and Information Engineering, Fuzhou University, Fuzhou, China


Abstract—Alpha-fetoprotein (AFP) is a useful diagnostic marker for the detection of a great number of infantile diseases, especially for some forms of malignant tumors and liver disorders. Although there are several methods for quantitative determination of AFP, compared with these methods, the gold immunochromatographic assay (GICA)has the advantages of easy to handle, low costs, quick, no requirement for skilled technicians and most important, well suitable for point-of-care measurements. However, GICA mostly has been used for qualitative or semi-quantitative detection visually. This paper presents a rapid quantitative determination method of AFP with GICA strip based on the reflective optical detection. Under the driving of the micro-stepper motor, we can get a curve of GICA strip signal distribution. Then after segmentation of the test line and control line using fuzzy c-means clustering algorithm, the features were extracted to be as the input features of Support Vector Regression (SVR) model. SVR was used for prediction of the AFP concentration. To evaluate the reliability and accuracy of GICA for fast quantitatively determination of AFP and its clinical value, the AFP concentration of specimen was tested by GICA and chemiluminescent immunoassay analyzer, and analyzed the coincident rate and correlation of these two methods. The experiment results demonstrated that the correlation coefficient results between the two methods was 0.961. The test results showed that the SVR model yields a good result and is proved to be appropriate in quantitative determination of AFP with GICA strip.

Index Terms—Gold immunochromatographic assay strip; Support Vector regression; alpha-fetoprotein (AFP).

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Cite: Haiyan Jiang, Min Du, Dongzhong Ke, "A Rapid Quantitative Determination Method of AFP Concentration with Gold Immunochromatographic Strip," Journal of Computers vol. 7, no. 12, pp. 2868-2875, 2012.

General Information

ISSN: 1796-203X
Abbreviated Title: J.Comput.
Frequency: Bimonthly
Editor-in-Chief: Prof. Liansheng Tan
Executive Editor: Ms. Nina Lee
Abstracting/ Indexing: DBLP, EBSCO,  ProQuest, INSPEC, ULRICH's Periodicals Directory, WorldCat, CNKI,etc
E-mail: jcp@iap.org
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